Microbiota community structure in traditional fermented milk dadiah in Indonesia: Insights from high-throughput 16S rRNA gene sequencing

A. Sukma1,2, H. Toh3, T.T.T. Nguyen1,4, N. Fitria5, I. Mimura1, R. Kaneko1, K. Arakawa1, H. Morita1
1 Graduate School of Environmental and Life Science, Okayama University, 1-1-1 Tsushima-naka, Okayama 700-8530, Japan
2 Faculty of Animal Science, Andalas University, Padang, Indonesia, Limau Manis, Pauh, Padang, West Sumatra 25163, Indonesia
3 Medical Institute of Bioregulation, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan
4 College of Agriculture and Forestry, Hue University, 102 Phung Hung street, Hue, Vietnam
5 Faculty of Science and Engineering PharmachoTheerapy, -Epidemiology and -Economics – Groningen, Research Institute of Pharmacy, Antonius Deusinglaan 9713 AV, Groningen, The Netherlands

Date submitted:30/05/2017        Date accepted: 08/08/2017                      Volume/Page(s): 71/1-3

Abstract
Dadiah is an Indonesian traditional fermented milk and is neither pasteurized nor boiled, but no food poisoning has been reported to date. Microbiota inhabiting dadiah has never been fully explored. In this study, we performed deep sequencing of 16S ribosomal RNA genes extracted from 11 dadiah samples and analyzed the dadiah microbiota at the genus level. We found that Lactococcus, Lactobacillus, and Leuconostoc were predominant among the dadiah microbiota. Unexpectedly, Klebsiella and Chryseobacterium, potential pathogens, were also found in some of the dadiah samples. There was little difference in the microbiota among samples taken from the same bamboo tube. In contrast, there were differences between the dadiah microbiota from different bamboo tubes, even those collected from the same sampling area. Furthermore, the composition of the dadiah microbiota showed large differences between sampling areas. We believe that our findings will lead to further improvement in the preparation of dadiah.

Key words: Dadiah, fermented buffalo milk, deep sequencing, 16S ribosomal RNA, microbiota

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